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recombinant mouse epha2 monoclonal antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology recombinant mouse epha2 monoclonal antibody
    FIGURE 3 EFNA1 chimeric antigen receptor (CAR)‐T cells effectively suppressed stem‐like pancreatic cancer cells. (A, B) The expression levels of the CSC markers CD133, CXCR4, OCT4, CD44, and CD24 on parental (WT) cells, and induced‐PANC1 CSC (A) and induced‐MIA‐PaCa2 CSC (B) were detected by real‐time quantitative PCR. (C) The protein expression levels of <t>EphA2</t> in parental (WT) cells and induced‐CSCs (PANC1‐CSC and MIA PaCa2‐CSC) were detected by western blot analysis. (D) The grayscale analysis of western blot analysis. (A–C) each include three technical replicates. The pictures showed are one example from these three technical replicates. (E) The cytotoxicity of NTD, MOCK, and EFNA1 CAR‐T cells against CSCs at an E:T ratio of 4:1 was measured using a luciferase cytotoxicity assay. (F–I) The release levels of IFN‐γ (F, G) and TNF‐α (H, I) were measured by enzyme‐linked immunosorbent assay after coculturing NTD, MOCK, and EFNA1 CAR‐T cells with CSCs for 24 h at an E:T ratio of 4:1. (E–I) comprises three biologic replicates and three technical replicates. An unpaired t‐test was used for the analysis of two groups, while a two‐tailed one‐way ANOVA was employed for three groups. Data are shown as mean ± SD; **p < 0.01, ***p < 0.001, ns, no significance.
    Recombinant Mouse Epha2 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+epha2+monoclonal+antibody/EphA2+Antibody/10__1002_slash_mog2__70010-206-19-24
    Average 95 stars, based on 293 article reviews
    recombinant mouse epha2 monoclonal antibody - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Ephrin A1 ligand‐based CAR‐T cells for immunotherapy of EphA2‐positive cancer"

    Article Title: Ephrin A1 ligand‐based CAR‐T cells for immunotherapy of EphA2‐positive cancer

    Journal: MedComm – Oncology

    doi: 10.1002/mog2.70010

    FIGURE 3 EFNA1 chimeric antigen receptor (CAR)‐T cells effectively suppressed stem‐like pancreatic cancer cells. (A, B) The expression levels of the CSC markers CD133, CXCR4, OCT4, CD44, and CD24 on parental (WT) cells, and induced‐PANC1 CSC (A) and induced‐MIA‐PaCa2 CSC (B) were detected by real‐time quantitative PCR. (C) The protein expression levels of EphA2 in parental (WT) cells and induced‐CSCs (PANC1‐CSC and MIA PaCa2‐CSC) were detected by western blot analysis. (D) The grayscale analysis of western blot analysis. (A–C) each include three technical replicates. The pictures showed are one example from these three technical replicates. (E) The cytotoxicity of NTD, MOCK, and EFNA1 CAR‐T cells against CSCs at an E:T ratio of 4:1 was measured using a luciferase cytotoxicity assay. (F–I) The release levels of IFN‐γ (F, G) and TNF‐α (H, I) were measured by enzyme‐linked immunosorbent assay after coculturing NTD, MOCK, and EFNA1 CAR‐T cells with CSCs for 24 h at an E:T ratio of 4:1. (E–I) comprises three biologic replicates and three technical replicates. An unpaired t‐test was used for the analysis of two groups, while a two‐tailed one‐way ANOVA was employed for three groups. Data are shown as mean ± SD; **p < 0.01, ***p < 0.001, ns, no significance.
    Figure Legend Snippet: FIGURE 3 EFNA1 chimeric antigen receptor (CAR)‐T cells effectively suppressed stem‐like pancreatic cancer cells. (A, B) The expression levels of the CSC markers CD133, CXCR4, OCT4, CD44, and CD24 on parental (WT) cells, and induced‐PANC1 CSC (A) and induced‐MIA‐PaCa2 CSC (B) were detected by real‐time quantitative PCR. (C) The protein expression levels of EphA2 in parental (WT) cells and induced‐CSCs (PANC1‐CSC and MIA PaCa2‐CSC) were detected by western blot analysis. (D) The grayscale analysis of western blot analysis. (A–C) each include three technical replicates. The pictures showed are one example from these three technical replicates. (E) The cytotoxicity of NTD, MOCK, and EFNA1 CAR‐T cells against CSCs at an E:T ratio of 4:1 was measured using a luciferase cytotoxicity assay. (F–I) The release levels of IFN‐γ (F, G) and TNF‐α (H, I) were measured by enzyme‐linked immunosorbent assay after coculturing NTD, MOCK, and EFNA1 CAR‐T cells with CSCs for 24 h at an E:T ratio of 4:1. (E–I) comprises three biologic replicates and three technical replicates. An unpaired t‐test was used for the analysis of two groups, while a two‐tailed one‐way ANOVA was employed for three groups. Data are shown as mean ± SD; **p < 0.01, ***p < 0.001, ns, no significance.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Luciferase, Cytotoxicity Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    FIGURE 4 EFNA1 chimeric antigen receptor (CAR)‐T cells exhibited effective antitumor activity in PANC1 xenograft mouse model. (A) Diagram illustrating the PANC1 pancreatic cancer mouse model. (B) Six‐week NCG mice were injected with PANC1‐luciferase cells (3 × 105/mouse) subcutaneously. MOCK T cells and EFNA1 CAR‐T cells (1 × 107/mouse: total number of T cells) were injected via the tail vein 4 days after PANC1‐luciferase injection. Tumor growth was monitored and evaluated based on total bioluminescence signals, n = 4. (C) The statistics of total radiance of the mice in MOCK and EFNA1 CAR groups. (D) Representative confocal microscopy images of the EphA2 expression in the tumors of MOCK and EFNA1 CAR groups, detected by immunofluorescence staining (scale bar = 50 μm). (E) The DNA copy numbers of MOCK T cells and EFNA1 CAR‐T cells in the peripheral blood were measured by real‐time quantitative PCR at different time points (n = 3). The experiments were conducted twice independently, yielding consistent results. Two groups were compared using an unpaired t‐test. The results are presented as the mean ± SD. *p < 0.05, **p < 0.01, ns, no significance.
    Figure Legend Snippet: FIGURE 4 EFNA1 chimeric antigen receptor (CAR)‐T cells exhibited effective antitumor activity in PANC1 xenograft mouse model. (A) Diagram illustrating the PANC1 pancreatic cancer mouse model. (B) Six‐week NCG mice were injected with PANC1‐luciferase cells (3 × 105/mouse) subcutaneously. MOCK T cells and EFNA1 CAR‐T cells (1 × 107/mouse: total number of T cells) were injected via the tail vein 4 days after PANC1‐luciferase injection. Tumor growth was monitored and evaluated based on total bioluminescence signals, n = 4. (C) The statistics of total radiance of the mice in MOCK and EFNA1 CAR groups. (D) Representative confocal microscopy images of the EphA2 expression in the tumors of MOCK and EFNA1 CAR groups, detected by immunofluorescence staining (scale bar = 50 μm). (E) The DNA copy numbers of MOCK T cells and EFNA1 CAR‐T cells in the peripheral blood were measured by real‐time quantitative PCR at different time points (n = 3). The experiments were conducted twice independently, yielding consistent results. Two groups were compared using an unpaired t‐test. The results are presented as the mean ± SD. *p < 0.05, **p < 0.01, ns, no significance.

    Techniques Used: Activity Assay, Injection, Luciferase, Confocal Microscopy, Expressing, Immunofluorescence, Staining, Real-time Polymerase Chain Reaction

    FIGURE 5 Effectiveness and safety of EFNA1 chimeric antigen receptor (CAR)‐T cells in mice. (A) EphA2 expression in mouse tumor cell lines (4T‐1 and Hepa1‐6) was detected by immunofluorescence staining, isotype as a negative control; scale bar = 20 μm. (B) Mean fluorescence intensity (MFI) of EphA2 staining per cell, n = 3. (A) and (B) each include three technical replicates. The pictures showed are one example from these three replicates. (C) The cytotoxicity of EFNA1 CAR‐T cells against 4T1 and Hepa1‐6 at an E:T ratio of 4:1 for 24 h was detected by luciferase‐based assay. (D, E) The IFN‐γ (D) and TNF‐α (E) release levels in the cocultured supernatants were assessed by enzyme‐linked immunosorbent assay. (C–E) comprise three biological and technical replicates. (F) Pathological analysis of the indicated organs in NCG mice was assessed after T‐cell injection for 56 days by hematoxylin and eosin (H&E) staining; scale bars = 50 μm. Three groups were analyzed by two‐tailed one‐way ANOVA. Data were plotted and are shown as mean ± SD; ***p < 0.001.
    Figure Legend Snippet: FIGURE 5 Effectiveness and safety of EFNA1 chimeric antigen receptor (CAR)‐T cells in mice. (A) EphA2 expression in mouse tumor cell lines (4T‐1 and Hepa1‐6) was detected by immunofluorescence staining, isotype as a negative control; scale bar = 20 μm. (B) Mean fluorescence intensity (MFI) of EphA2 staining per cell, n = 3. (A) and (B) each include three technical replicates. The pictures showed are one example from these three replicates. (C) The cytotoxicity of EFNA1 CAR‐T cells against 4T1 and Hepa1‐6 at an E:T ratio of 4:1 for 24 h was detected by luciferase‐based assay. (D, E) The IFN‐γ (D) and TNF‐α (E) release levels in the cocultured supernatants were assessed by enzyme‐linked immunosorbent assay. (C–E) comprise three biological and technical replicates. (F) Pathological analysis of the indicated organs in NCG mice was assessed after T‐cell injection for 56 days by hematoxylin and eosin (H&E) staining; scale bars = 50 μm. Three groups were analyzed by two‐tailed one‐way ANOVA. Data were plotted and are shown as mean ± SD; ***p < 0.001.

    Techniques Used: Expressing, Immunofluorescence, Staining, Negative Control, Fluorescence, Luciferase, Enzyme-linked Immunosorbent Assay, Injection, Two Tailed Test

    Related Articles

    Recombinant:

    Article Title: Ephrin A1 ligand‐based CAR‐T cells for immunotherapy of EphA2‐positive cancer
    Article Snippet: .. The study utilized mouse β‐actin monoclonal antibody (Santa Cruz, 1:1000, SC‐47778), rabbit GAPDH monoclonal antibody (abcam, 1:1000, ab9484), and recombinant mouse EphA2 monoclonal antibody (Santa Cruz 1:1000, SC‐398832). ..



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    recombinant mouse epha2 monoclonal antibody  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology recombinant mouse epha2 monoclonal antibody
    FIGURE 3 EFNA1 chimeric antigen receptor (CAR)‐T cells effectively suppressed stem‐like pancreatic cancer cells. (A, B) The expression levels of the CSC markers CD133, CXCR4, OCT4, CD44, and CD24 on parental (WT) cells, and induced‐PANC1 CSC (A) and induced‐MIA‐PaCa2 CSC (B) were detected by real‐time quantitative PCR. (C) The protein expression levels of <t>EphA2</t> in parental (WT) cells and induced‐CSCs (PANC1‐CSC and MIA PaCa2‐CSC) were detected by western blot analysis. (D) The grayscale analysis of western blot analysis. (A–C) each include three technical replicates. The pictures showed are one example from these three technical replicates. (E) The cytotoxicity of NTD, MOCK, and EFNA1 CAR‐T cells against CSCs at an E:T ratio of 4:1 was measured using a luciferase cytotoxicity assay. (F–I) The release levels of IFN‐γ (F, G) and TNF‐α (H, I) were measured by enzyme‐linked immunosorbent assay after coculturing NTD, MOCK, and EFNA1 CAR‐T cells with CSCs for 24 h at an E:T ratio of 4:1. (E–I) comprises three biologic replicates and three technical replicates. An unpaired t‐test was used for the analysis of two groups, while a two‐tailed one‐way ANOVA was employed for three groups. Data are shown as mean ± SD; **p < 0.01, ***p < 0.001, ns, no significance.
    Recombinant Mouse Epha2 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+epha2+monoclonal+antibody/EphA2+Antibody/10__1002_slash_mog2__70010-206-19-24
    Average 95 stars, based on 1 article reviews
    recombinant mouse epha2 monoclonal antibody - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

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    FIGURE 3 EFNA1 chimeric antigen receptor (CAR)‐T cells effectively suppressed stem‐like pancreatic cancer cells. (A, B) The expression levels of the CSC markers CD133, CXCR4, OCT4, CD44, and CD24 on parental (WT) cells, and induced‐PANC1 CSC (A) and induced‐MIA‐PaCa2 CSC (B) were detected by real‐time quantitative PCR. (C) The protein expression levels of EphA2 in parental (WT) cells and induced‐CSCs (PANC1‐CSC and MIA PaCa2‐CSC) were detected by western blot analysis. (D) The grayscale analysis of western blot analysis. (A–C) each include three technical replicates. The pictures showed are one example from these three technical replicates. (E) The cytotoxicity of NTD, MOCK, and EFNA1 CAR‐T cells against CSCs at an E:T ratio of 4:1 was measured using a luciferase cytotoxicity assay. (F–I) The release levels of IFN‐γ (F, G) and TNF‐α (H, I) were measured by enzyme‐linked immunosorbent assay after coculturing NTD, MOCK, and EFNA1 CAR‐T cells with CSCs for 24 h at an E:T ratio of 4:1. (E–I) comprises three biologic replicates and three technical replicates. An unpaired t‐test was used for the analysis of two groups, while a two‐tailed one‐way ANOVA was employed for three groups. Data are shown as mean ± SD; **p < 0.01, ***p < 0.001, ns, no significance.

    Journal: MedComm – Oncology

    Article Title: Ephrin A1 ligand‐based CAR‐T cells for immunotherapy of EphA2‐positive cancer

    doi: 10.1002/mog2.70010

    Figure Lengend Snippet: FIGURE 3 EFNA1 chimeric antigen receptor (CAR)‐T cells effectively suppressed stem‐like pancreatic cancer cells. (A, B) The expression levels of the CSC markers CD133, CXCR4, OCT4, CD44, and CD24 on parental (WT) cells, and induced‐PANC1 CSC (A) and induced‐MIA‐PaCa2 CSC (B) were detected by real‐time quantitative PCR. (C) The protein expression levels of EphA2 in parental (WT) cells and induced‐CSCs (PANC1‐CSC and MIA PaCa2‐CSC) were detected by western blot analysis. (D) The grayscale analysis of western blot analysis. (A–C) each include three technical replicates. The pictures showed are one example from these three technical replicates. (E) The cytotoxicity of NTD, MOCK, and EFNA1 CAR‐T cells against CSCs at an E:T ratio of 4:1 was measured using a luciferase cytotoxicity assay. (F–I) The release levels of IFN‐γ (F, G) and TNF‐α (H, I) were measured by enzyme‐linked immunosorbent assay after coculturing NTD, MOCK, and EFNA1 CAR‐T cells with CSCs for 24 h at an E:T ratio of 4:1. (E–I) comprises three biologic replicates and three technical replicates. An unpaired t‐test was used for the analysis of two groups, while a two‐tailed one‐way ANOVA was employed for three groups. Data are shown as mean ± SD; **p < 0.01, ***p < 0.001, ns, no significance.

    Article Snippet: The study utilized mouse β‐actin monoclonal antibody (Santa Cruz, 1:1000, SC‐47778), rabbit GAPDH monoclonal antibody (abcam, 1:1000, ab9484), and recombinant mouse EphA2 monoclonal antibody (Santa Cruz 1:1000, SC‐398832).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Luciferase, Cytotoxicity Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    FIGURE 4 EFNA1 chimeric antigen receptor (CAR)‐T cells exhibited effective antitumor activity in PANC1 xenograft mouse model. (A) Diagram illustrating the PANC1 pancreatic cancer mouse model. (B) Six‐week NCG mice were injected with PANC1‐luciferase cells (3 × 105/mouse) subcutaneously. MOCK T cells and EFNA1 CAR‐T cells (1 × 107/mouse: total number of T cells) were injected via the tail vein 4 days after PANC1‐luciferase injection. Tumor growth was monitored and evaluated based on total bioluminescence signals, n = 4. (C) The statistics of total radiance of the mice in MOCK and EFNA1 CAR groups. (D) Representative confocal microscopy images of the EphA2 expression in the tumors of MOCK and EFNA1 CAR groups, detected by immunofluorescence staining (scale bar = 50 μm). (E) The DNA copy numbers of MOCK T cells and EFNA1 CAR‐T cells in the peripheral blood were measured by real‐time quantitative PCR at different time points (n = 3). The experiments were conducted twice independently, yielding consistent results. Two groups were compared using an unpaired t‐test. The results are presented as the mean ± SD. *p < 0.05, **p < 0.01, ns, no significance.

    Journal: MedComm – Oncology

    Article Title: Ephrin A1 ligand‐based CAR‐T cells for immunotherapy of EphA2‐positive cancer

    doi: 10.1002/mog2.70010

    Figure Lengend Snippet: FIGURE 4 EFNA1 chimeric antigen receptor (CAR)‐T cells exhibited effective antitumor activity in PANC1 xenograft mouse model. (A) Diagram illustrating the PANC1 pancreatic cancer mouse model. (B) Six‐week NCG mice were injected with PANC1‐luciferase cells (3 × 105/mouse) subcutaneously. MOCK T cells and EFNA1 CAR‐T cells (1 × 107/mouse: total number of T cells) were injected via the tail vein 4 days after PANC1‐luciferase injection. Tumor growth was monitored and evaluated based on total bioluminescence signals, n = 4. (C) The statistics of total radiance of the mice in MOCK and EFNA1 CAR groups. (D) Representative confocal microscopy images of the EphA2 expression in the tumors of MOCK and EFNA1 CAR groups, detected by immunofluorescence staining (scale bar = 50 μm). (E) The DNA copy numbers of MOCK T cells and EFNA1 CAR‐T cells in the peripheral blood were measured by real‐time quantitative PCR at different time points (n = 3). The experiments were conducted twice independently, yielding consistent results. Two groups were compared using an unpaired t‐test. The results are presented as the mean ± SD. *p < 0.05, **p < 0.01, ns, no significance.

    Article Snippet: The study utilized mouse β‐actin monoclonal antibody (Santa Cruz, 1:1000, SC‐47778), rabbit GAPDH monoclonal antibody (abcam, 1:1000, ab9484), and recombinant mouse EphA2 monoclonal antibody (Santa Cruz 1:1000, SC‐398832).

    Techniques: Activity Assay, Injection, Luciferase, Confocal Microscopy, Expressing, Immunofluorescence, Staining, Real-time Polymerase Chain Reaction

    FIGURE 5 Effectiveness and safety of EFNA1 chimeric antigen receptor (CAR)‐T cells in mice. (A) EphA2 expression in mouse tumor cell lines (4T‐1 and Hepa1‐6) was detected by immunofluorescence staining, isotype as a negative control; scale bar = 20 μm. (B) Mean fluorescence intensity (MFI) of EphA2 staining per cell, n = 3. (A) and (B) each include three technical replicates. The pictures showed are one example from these three replicates. (C) The cytotoxicity of EFNA1 CAR‐T cells against 4T1 and Hepa1‐6 at an E:T ratio of 4:1 for 24 h was detected by luciferase‐based assay. (D, E) The IFN‐γ (D) and TNF‐α (E) release levels in the cocultured supernatants were assessed by enzyme‐linked immunosorbent assay. (C–E) comprise three biological and technical replicates. (F) Pathological analysis of the indicated organs in NCG mice was assessed after T‐cell injection for 56 days by hematoxylin and eosin (H&E) staining; scale bars = 50 μm. Three groups were analyzed by two‐tailed one‐way ANOVA. Data were plotted and are shown as mean ± SD; ***p < 0.001.

    Journal: MedComm – Oncology

    Article Title: Ephrin A1 ligand‐based CAR‐T cells for immunotherapy of EphA2‐positive cancer

    doi: 10.1002/mog2.70010

    Figure Lengend Snippet: FIGURE 5 Effectiveness and safety of EFNA1 chimeric antigen receptor (CAR)‐T cells in mice. (A) EphA2 expression in mouse tumor cell lines (4T‐1 and Hepa1‐6) was detected by immunofluorescence staining, isotype as a negative control; scale bar = 20 μm. (B) Mean fluorescence intensity (MFI) of EphA2 staining per cell, n = 3. (A) and (B) each include three technical replicates. The pictures showed are one example from these three replicates. (C) The cytotoxicity of EFNA1 CAR‐T cells against 4T1 and Hepa1‐6 at an E:T ratio of 4:1 for 24 h was detected by luciferase‐based assay. (D, E) The IFN‐γ (D) and TNF‐α (E) release levels in the cocultured supernatants were assessed by enzyme‐linked immunosorbent assay. (C–E) comprise three biological and technical replicates. (F) Pathological analysis of the indicated organs in NCG mice was assessed after T‐cell injection for 56 days by hematoxylin and eosin (H&E) staining; scale bars = 50 μm. Three groups were analyzed by two‐tailed one‐way ANOVA. Data were plotted and are shown as mean ± SD; ***p < 0.001.

    Article Snippet: The study utilized mouse β‐actin monoclonal antibody (Santa Cruz, 1:1000, SC‐47778), rabbit GAPDH monoclonal antibody (abcam, 1:1000, ab9484), and recombinant mouse EphA2 monoclonal antibody (Santa Cruz 1:1000, SC‐398832).

    Techniques: Expressing, Immunofluorescence, Staining, Negative Control, Fluorescence, Luciferase, Enzyme-linked Immunosorbent Assay, Injection, Two Tailed Test